Production, purification, and characterization of alkaline protease from Aspergillus flavus and its compatibility with commercial detergents
نویسندگان
چکیده
منابع مشابه
Isolation, Production, Purification, Assay and Characterization of Alkaline Protease Enzyme from Aspergillus niger and its Compatibility with Commercial Detergents
Aspergillus niger is a highly potent fungus used in the production of alkaline protease. Extra cellular alkaline protease was purified from A. niger in a twostep procedure involving ammonium sulphate precipitation and Sephadex G100 column chromatography. The molecular mass of the enzyme was determined to be 60 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The ...
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An alkalophilic fungal strain, Aspergillus versicolor PF/F/107 isolated from poultry farm produced alkaline protease at 40C and 9.0 pH. Protease showed compatibility with commercial detergents and retained 50-76% of its original activity at 40C in the presence of detergents in the following order: Ghadi, Ujalla, Surf excel, Ariel and Tide indicating its suitability for application in detergen...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملProduction, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation
Protease production under solid state fermentation (SSF) was investigated using isolated Aspergillus flavus. Different agroindustrial waste products were evaluated to check the possibility of potential utilization of substrates in SSF for protease production by Aspergillus flavus using wheat bran as a substrate. The results showed that the optimum conditions for maximum protease production were...
متن کاملPurification, characterization and crystallization of an extracellular alkaline protease from Aspergillus nidulans HA-10.
Aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. Extracellular alkaline protease was purified from A. nidulans in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-100 column chromatography. The molecular mass of the enzyme was determined to be 42 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelati...
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ژورنال
عنوان ژورنال: BioResources
سال: 2020
ISSN: 1930-2126,1930-2126
DOI: 10.15376/biores.16.1.291-301